CompoundList
Sign in
Chromatography and spectroscopy reagents

Dinitrofluorobenzene

Also known as 2,4-Dinitrofluorobenzene, 1-Fluoro-2,4-dinitrobenzene, FDNB

CAS 70-34-8Formula C6H3FN2O4Molar mass 186.10 g/molEC 200-734-3
GHS signal word: Danger. Pictograms: Corrosive, Acute toxicity (severe), Harmful or irritant, Serious health hazard 60 suppliers listed
Get quotes Compare 60 suppliers
Dinitrofluorobenzene chemical structure, C6H3FN2O4, CAS 70-34-8
2D chemical structure

Key facts

What buyers check first

All properties
Appearance
Pale yellow crystals from ether
Melting point
25.8 °C
Boiling point
296 °C
Density
1.4718 g/cm³ at 54 °C
Flash point
>112.8
HS code
290420
Category
Chromatography and spectroscopy reagents
UN number
UN 2811
Illustrative photo, off-white crystalline powder in an HDPE jar on a laboratory bench
Typical packagingIllustrative packaging image, not the actual product

What is Dinitrofluorobenzene?

Plain description, key identifiers and synonyms

1-fluoro-2,4-dinitrobenzene is the organofluorine compound that is benzene with a fluoro substituent at the 1-position and two nitro substituents in the 2- and 4-positions. It has a role as an agrochemical, an allergen, a chromatographic reagent, a protein-sequencing agent, an EC 2.7.3.2 (creatine kinase) inhibitor and a spectrophotometric reagent. It is a C-nitro compound and an organofluorine compound.

Dinitrofluorobenzene is a chemical used for polypeptide sequencing.

Identifiers

Registry numbers, names and line notations, tap to copy

IUPAC name
1-fluoro-2,4-dinitrobenzene
CAS number
70-34-8 CAS number cross-checked
EC number
200-734-3
Molecular formula
C6H3FN2O4
UNII
D241E059U6
MDL number
MFCD00007056
InChIKey
LOTKRQAVGJMPNV-UHFFFAOYSA-N
InChI
InChI=1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H
SMILES (canonical and isomeric)
C1=CC(=C(C=C1[N+](=O)[O-])[N+](=O)[O-])F

Synonyms

  • 2,4-Dinitrofluorobenzene
  • 1-Fluoro-2,4-dinitrobenzene
  • FDNB
  • DNFB
  • 2,4-Dinitro-1-fluorobenzene
  • Sanger's Reagent
  • 2,4-Dinitrophenyl fluoride
  • Fluorodinitrobenzene
  • 2,4-Dinitrobenzene fluoride
  • 2,4-Dinitrobenzenefluoride
  • Fluoro-2,4-dinitrobenzene
  • Benzene, 1-fluoro-2,4-dinitro-
  • 2,4-DNFB
  • Benzene, 2,4-dinitro-1-fluoro-
  • 1,2,4-Fluorodinitrobenzene
  • 4-Fluoro-1,3-dinitrobenzene
  • 1 Fluoro 2,4 dinitrobenzene
  • 2,4-nitrofluorobenzene
  • 1-fluoro-2,4-dinitro-benzene
  • DFB (VAN)
  • Fluorodinitrobenzene (VAN)
  • 2,4-dinitrofluorbenzene
  • 2,4-dinitroflurobenzene
  • 2.4-dinitrofluorobenzene
  • 1,4-Fluorodinitrobenzene
Show all 36 synonyms
  • 2,4-dinitrophenylfluoride
  • 2,4-dinitro-1fluorbenzen
  • 2,4-dinitro-fluorobenzene
  • 2,4-dinitrofluoro-benzene
  • 2,4-dinitro-1-fluorbenzen
  • 1-fluoro-2,4-dinitrobenzen
  • 4-05-00-00742 (Beilstein Handbook Reference)
  • Benzene,4-dinitro-1-fluoro-
  • 1-Fluoro-2,4-dinitrobenzene, >=99%
  • 1-Fluoro-2,4-dinitrobenzene: Sanger's reagent
  • 1-Fluoro-2,4-dinitrobenzene, >=99.0% (GC)

Physical and chemical properties

Measured values and descriptors computed from the structure

Physical and chemical properties

Measured values, check the supplier specification for your grade

Appearance
Pale yellow crystals from etherexp.
Melting point
25.8 °Cexp.
Boiling point
296 °Cexp.
Density
1.4718 g/cm³ at 54 °Cexp.
Flash point
>112.8exp.
Solubility
In water, 400 mg/L at 25 °Cexp.

Values vary by grade. Ask suppliers for the COA or TDS of the batch you buy.

Computed descriptors

Calculated from the structure, useful for screening and formulation

Molecular weight
186.10 g/molcalc.
Exact mass
186.0077 Dacalc.
LogP (octanol/water)
1.64calc.
Topological polar surface area
86.28 Ųcalc.
Hydrogen bond donors
0calc.
Hydrogen bond acceptors
4calc.
Rotatable bonds
2calc.
Heavy atoms
13calc.
Rings
1calc.
Aromatic rings
1calc.
Fraction sp3 carbons
0calc.
Formal charge
0calc.
Stereocentres
0calc.
Lipinski rule of five
Passes (0 violations)calc.

Calculated for screening. Confirm critical figures with the supplier.

Murcko scaffold

The ring system that remains when every side chain is removed

Murcko scaffold of this chemical, c1ccccc1

c1ccccc1

1 ring, 6 atoms, the Benzene ring system.

485,677 other chemicals share this scaffold

Safety and hazards

GHS classification summary, not a safety data sheet

GHS signal word
Danger
GHS05: Corrosive
Corrosive
GHS06: Acute toxicity (severe)
Acute toxicity (severe)
GHS07: Harmful or irritant
Harmful or irritant
GHS08: Serious health hazard
Serious health hazard

Hazard statements

  • H301Toxic if swallowed.
  • H302Harmful if swallowed.
  • H311Toxic in contact with skin.
  • H315Causes skin irritation.
  • H317May cause an allergic skin reaction.
  • H318Causes serious eye damage.
  • H319Causes serious eye irritation.
  • H330Fatal if inhaled.
  • H335May cause respiratory irritation.
  • H373May cause damage to organs through prolonged or repeated exposure.
Precautionary statements (30)
  • P260Do not breathe dust, fume, gas, mist, vapours or spray.
  • P261Avoid breathing dust, fume, gas, mist, vapours or spray.
  • P262Do not get in eyes, on skin, or on clothing.
  • P264Wash skin thoroughly after handling.
  • P264 + P265Wash skin thoroughly after handling. Do not touch eyes.
  • P270Do not eat, drink or smoke when using this product.
  • P271Use only outdoors or in a well-ventilated area.
  • P272Contaminated work clothing should not be allowed out of the workplace.
  • P280Wear protective gloves, protective clothing, eye protection and face protection.
  • P284In case of inadequate ventilation wear respiratory protection.
  • P301 + P316If swallowed: Get emergency medical help immediately.
  • P301 + P317If swallowed: Get medical help.
  • P302 + P352If on skin: Wash with plenty of water.
  • P304 + P340If inhaled: Remove person to fresh air and keep comfortable for breathing.
  • P305 + P351 + P338If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P305 + P354 + P338If in eyes: Immediately rinse with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P316Get emergency medical help immediately.
  • P317Get medical help.
  • P319Get medical help if you feel unwell.
  • P320Specific treatment is urgent (see supplemental first aid instructions on the label).
  • P321Specific treatment (see supplemental first aid instructions on the label).
  • P330Rinse mouth.
  • P332 + P317If skin irritation occurs: Get medical help.
  • P333 + P317If skin irritation or rash occurs: Get medical help.
  • P337 + P317If eye irritation persists: Get medical help.
  • P361 + P364Take off immediately all contaminated clothing. And wash it before reuse.
  • P362 + P364Take off contaminated clothing. And wash it before reuse.
  • P403 + P233Store in a well-ventilated place. Keep container tightly closed.
  • P405Store locked up.
  • P501Dispose of contents and container in accordance with local regulations.
UN number
UN 2811

GHS classification summary, not a safety data sheet (SDS). Classifications can differ by supplier, grade and concentration. Always check the supplier's SDS.

Full safety data and first aid

Dinitrofluorobenzene suppliers

Sponsored listings first, then the documented ranking: membership, verification, replies, completeness, freshness and ratings

All 60 suppliers
30 of 30 offers

Suppliers marked Unclaimed profile have not claimed their profile yet. They are not verified by CompoundList, their offers are price on request and details may have changed, so please confirm them with the supplier. Company representatives can claim a profile with a business email on the company's own website domain. Our team verifies each claim and sends the result by email.

Where suppliers are based

60 suppliers in 13 countries

Send one request to many suppliers

Choose up to 10 suppliers or let us match your request. Compare quotes side by side, free.

Choose suppliersPost a request

Dinitrofluorobenzene price

Indicative price range per kg from live supplier offers

No published prices right now

Suppliers quote this chemical on request. Send one free request and compare quotes side by side.

Get latest price

Uses and applications

What the substance is used for, by industry and function

Reagent for labeling a terminal amino acid group, in modified Wohl degradations of aldoses. As hapten.

Alkylating agent, reagent in elucidating amino acid sequence in proteins.

Roles

  • aetiopathogenetic role
  • agrochemical
  • allergen
  • chromatographic reagent
  • enzyme inhibitor
  • protein-sequencing agent
  • reagent
  • spectrophotometric reagent

Pharmacology

Dinitrofluorobenzene is an aromatic hydrocarbon composed of a benzene ring linked to two nitro groups and one fluoride group that is used for N-terminal amino acid sequencing by the Sanger method. In vitro, dinitrofluorobenzene (DNFB) reacts with the amine group in amino acids to produce dinitrophenyl (DNP)-amino acids. Then the sample is subjected to acid hydrolysis. Under these conditions, the peptide bonds are broken but the DNP-amino acid bonds remain intact. The DNP-amino acids can be recovered and their identities can be determined through the use of various chromatography techniques. This reagent can also be used to distinguish the reduced and oxidized forms of glutathione and cysteine.

How it is made

In commercial batch or semicontinuous operations, (such as) the Halex process, potassium fluoride and the activated chloroaromatic compound are thoroughly mixed (1:1) with a large volume of an aprotic solvent (dimethyl sulfoxide or sulfolane) and are heated to 150-250 °C. The reaction is 90% complete within 48 hr. Removal of KCl and distillation of the product present no difficulty ... The following commercially important ... (intermediate is) obtained by the Halex process: ... 1- fluoro-2,4-dinitrobenzene from 1- chloro-2,4-dinitrobenzene.

Regulatory status

Inventories, regulations and trade controls that list this substance

United States

  • TSCA inventory (USA)
    Status: Active in US commerce

HS code and trade data

Customs classification and reported world trade statistics

Global trade

World import and export statistics for the HS code Dinitrofluorobenzene is traded under.

HS 2904.20Indicative HS code

Derivatives containing only nitro or only nitroso groups

World exports in 2024
$440M
+46.4% vs 2023, imports $463M
2019 to 2024
Top exporters
  1. Germany27.6%
  2. Poland17.1%
  3. Portugal16.0%
Top importers
  1. Belgium34.0%
  2. United States11.0%
  3. Germany9.1%
Reported customs values in USD, not prices.Full trade data

The HS code shown for Dinitrofluorobenzene is indicative (it was assigned by structure rules). Confirm the classification with your customs broker before shipping.

Frequently asked questions

Quick answers about Dinitrofluorobenzene, based on the data on this page

What is Dinitrofluorobenzene?

1-fluoro-2,4-dinitrobenzene is the organofluorine compound that is benzene with a fluoro substituent at the 1-position and two nitro substituents in the 2- and 4-positions.

What is the CAS number of Dinitrofluorobenzene?

The CAS registry number of Dinitrofluorobenzene is 70-34-8.

What is the molecular formula and weight of Dinitrofluorobenzene?

The molecular formula of Dinitrofluorobenzene is C6H3FN2O4 and its molar mass is 186.10 g/mol.

What is Dinitrofluorobenzene used for?

Reagent for labeling a terminal amino acid group, in modified Wohl degradations of aldoses. As hapten. Alkylating agent, reagent in elucidating amino acid sequence in proteins.

Is Dinitrofluorobenzene hazardous?

Dinitrofluorobenzene carries the GHS signal word "Danger". Main hazards: toxic if swallowed, harmful if swallowed, toxic in contact with skin. This summary is not an SDS, always read the supplier safety data sheet.

What is the HS code of Dinitrofluorobenzene?

Dinitrofluorobenzene is usually classified under HS code 290420 (Derivatives containing only nitro or only nitroso groups). This is indicative, confirm it with your customs broker before shipping.

Where can I buy Dinitrofluorobenzene?

CompoundList lists 60 suppliers of Dinitrofluorobenzene in United States, China, Germany, United Kingdom and 9 more. Send one free request to get and compare quotes. Your contact details stay private until you accept a quote.

Where can I buy Dinitrofluorobenzene in India?

4 suppliers in India list Dinitrofluorobenzene on CompoundList. Filter the suppliers list by country and send a request to compare prices, grades and delivery times.

What is the price of Dinitrofluorobenzene?

Suppliers quote Dinitrofluorobenzene on request because prices depend on grade, purity, quantity and destination. A free request on CompoundList gets you several quotes to compare.

Guides for buyers

Practical guides that help when you source, ship or handle Dinitrofluorobenzene

Get quotes for Dinitrofluorobenzene

Post one free request with your grade, quantity and destination. Matching suppliers send quotes you can compare side by side.
Post a request Sell Dinitrofluorobenzene
Data last updated 7 Oct 2026. Details may have changed, please confirm them with the supplier. Report an error on this page
Embed this structure on your websiteShow code

Paste this code into your page to show the 2D structure of Dinitrofluorobenzene with a 3D view, its CAS number and a link back to this page. Free to use with the link kept.

<iframe src="https://www.compoundlist.com/embed/structure/dinitrofluorobenzene-70-34-8" title="Dinitrofluorobenzene structure on CompoundList" width="360" height="420" style="border:1px solid #E7E2DA;border-radius:12px;max-width:100%" loading="lazy" referrerpolicy="strict-origin-when-cross-origin"></iframe>
Preview the embed

Explore more in Chromatography and spectroscopy reagents or HS code 290420

60 suppliers
Free quotes, contact stays private
Get quotes