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Indicators and stains

3,3',5,5'-Tetramethylbenzidine

Also known as 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine, 3,5,3',5'-Tetramethylbenzidine, Colorburst blue

CAS 54827-17-7Formula C16H20N2Molar mass 240.35 g/molEC 259-364-6
GHS signal word: Warning. Pictograms: Harmful or irritant, Serious health hazard 96 suppliers listed
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3,3',5,5'-Tetramethylbenzidine chemical structure, C16H20N2, CAS 54827-17-7
2D chemical structure

Key facts

What buyers check first

All properties
Physical form
Crystals
Melting point
168.5 °C
Solubility
Less than 1 mg/mL at 68 °F
HS code
292159
Category
Indicators and stains
Illustrative photo, a shrink wrapped pallet of kraft paper sacks in a chemical plant
Typical packagingIllustrative packaging image, not the actual product

What is 3,3',5,5'-Tetramethylbenzidine?

Plain description, key identifiers and synonyms

3,3',5,5'-tetramethylbenzidine appears as pale yellow crystals or off-white powder. (NTP, 1992)

Identifiers

Registry numbers, names and line notations, tap to copy

IUPAC name
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
CAS number
54827-17-7 CAS number cross-checked
EC number
259-364-6
Molecular formula
C16H20N2
UNII
3B3T5CB8EO
MDL number
MFCD00007748
InChIKey
UAIUNKRWKOVEES-UHFFFAOYSA-N
InChI
InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
SMILES (canonical and isomeric)
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

Synonyms

  • 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine
  • 3,5,3',5'-Tetramethylbenzidine
  • Colorburst blue
  • 3,3',5,5'-Tetramethyl-(1,1'-biphenyl)-4,4'-diamine
  • (1,1'-Biphenyl)-4,4'-diamine, 3,3',5,5'-tetramethyl-
  • Potassium-blue max
  • 3,3',5,5'-Tetramethyl(1,1'-biphenyl)-4,4'-diamine
  • [1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-
  • 3,3',5,5'-Tetramethyl[1,1'-biphenyl]-4,4'-diamine
  • tetramethyl benzidine
  • 3,3',5,5'-TMP
  • TMB
  • 3,3',5,5-tetramethylbenzidine
  • 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
  • 4,4'-Bi-2,6-xylidine
  • 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine
  • 3,3',5,5'-tetramethylbenzidine (tmb)
  • bm blue
  • 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl
  • 3,3',5,5'-Tetramethylbenzidine, >=98.0% (NT)
  • 3,3',5,5'-tetramethyl benzidine
  • 3,3 inverted exclamation marka,5,5 inverted exclamation marka-Tetramethylbenzidine
  • 3,3',5',5-tetramethylbenzidine
  • Sure Blue TMB
  • 3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate
Show all 35 synonyms
  • TMB Blotting Plus
  • 4-(4-Amino-3,5-dimethyl-phenyl)-2,6-dimethyl-aniline
  • TMB substrate pound>>TMB
  • 3,3'5,5'-tetramethylbenzidine
  • 3,3',5,5'-tetramethylbenzidin
  • 3,3,5',5'-Tetramethylbenzidine
  • 3,3',5,5'-tetramethyl-benzidine
  • TMB, 3,3',5,5'-Tetramethylbenzidine
  • 3,3',5,5'-Tetramethylbenzidine, Reagent
  • TMB [for ELISA] (Ready-to-use solution)

Physical and chemical properties

Measured values and descriptors computed from the structure

Physical and chemical properties

Measured values, check the supplier specification for your grade

Physical form
Crystalsexp.
Melting point
168.5 °Cexp.
Solubility
Less than 1 mg/mL at 68 °Fexp.

Values vary by grade. Ask suppliers for the COA or TDS of the batch you buy.

Computed descriptors

Calculated from the structure, useful for screening and formulation

Molecular weight
240.35 g/molcalc.
Exact mass
240.1626 Dacalc.
LogP (octanol/water)
3.75calc.
Topological polar surface area
52.04 Ųcalc.
Hydrogen bond donors
2calc.
Hydrogen bond acceptors
2calc.
Rotatable bonds
1calc.
Heavy atoms
18calc.
Rings
2calc.
Aromatic rings
2calc.
Fraction sp3 carbons
0.25calc.
Formal charge
0calc.
Stereocentres
0calc.
Lipinski rule of five
Passes (0 violations)calc.

Calculated for screening. Confirm critical figures with the supplier.

Murcko scaffold

The ring system that remains when every side chain is removed

Murcko scaffold of this chemical, c1ccc(-c2ccccc2)cc1

c1ccc(-c2ccccc2)cc1

2 rings, 12 atoms, the Biphenyl ring system.

32,228 other chemicals share this scaffold

Safety and hazards

GHS classification summary, not a safety data sheet

GHS signal word
Warning
GHS07: Harmful or irritant
Harmful or irritant
GHS08: Serious health hazard
Serious health hazard

Hazard statements

  • H302Harmful if swallowed.
  • H315Causes skin irritation.
  • H319Causes serious eye irritation.
  • H335May cause respiratory irritation.
  • H341Suspected of causing genetic defects.
Precautionary statements (21)
  • P203Obtain, read and follow all safety instructions before use.
  • P261Avoid breathing dust, fume, gas, mist, vapours or spray.
  • P264Wash skin thoroughly after handling.
  • P264 + P265Wash skin thoroughly after handling. Do not touch eyes.
  • P270Do not eat, drink or smoke when using this product.
  • P271Use only outdoors or in a well-ventilated area.
  • P280Wear protective gloves, protective clothing, eye protection and face protection.
  • P301 + P317If swallowed: Get medical help.
  • P302 + P352If on skin: Wash with plenty of water.
  • P304 + P340If inhaled: Remove person to fresh air and keep comfortable for breathing.
  • P305 + P351 + P338If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P318If exposed or concerned, get medical advice.
  • P319Get medical help if you feel unwell.
  • P321Specific treatment (see supplemental first aid instructions on the label).
  • P330Rinse mouth.
  • P332 + P317If skin irritation occurs: Get medical help.
  • P337 + P317If eye irritation persists: Get medical help.
  • P362 + P364Take off contaminated clothing. And wash it before reuse.
  • P403 + P233Store in a well-ventilated place. Keep container tightly closed.
  • P405Store locked up.
  • P501Dispose of contents and container in accordance with local regulations.

GHS classification summary, not a safety data sheet (SDS). Classifications can differ by supplier, grade and concentration. Always check the supplier's SDS.

Full safety data and first aid

3,3',5,5'-Tetramethylbenzidine suppliers

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All 96 suppliers
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Where suppliers are based

96 suppliers in 14 countries

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3,3',5,5'-Tetramethylbenzidine price

Indicative price range per kg from live supplier offers

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Uses and applications

What the substance is used for, by industry and function

Clinical reagent for testing blood.

Pharmacological actions

  • Chromogenic Compounds

Regulatory status

Inventories, regulations and trade controls that list this substance

United States

  • TSCA inventory (USA)
    Status: Active in US commerce

HS code and trade data

Customs classification and reported world trade statistics

Global trade

World import and export statistics for the HS code 3,3',5,5'-Tetramethylbenzidine is traded under.

HS 2921.59Indicative HS code

Aromatic polyamines and their derivatives, salts thereof, other

World exports in 2024
$510M
−0.4% vs 2023, imports $488M
2019 to 2024
Top exporters
  1. China26.6%
  2. Japan16.6%
  3. India * partner reported12.4%
Top importers
  1. United States17.9%
  2. Germany11.6%
  3. South Korea7.4%

* Partner reported figures, a lower bound.

Reported customs values in USD, not prices.Full trade data

The HS code shown for 3,3',5,5'-Tetramethylbenzidine is indicative (it was assigned by structure rules). Confirm the classification with your customs broker before shipping.

Frequently asked questions

Quick answers about 3,3',5,5'-Tetramethylbenzidine, based on the data on this page

What is 3,3',5,5'-Tetramethylbenzidine?

3,3',5,5'-tetramethylbenzidine appears as pale yellow crystals or off-white powder. (NTP, 1992)

What is the CAS number of 3,3',5,5'-Tetramethylbenzidine?

The CAS registry number of 3,3',5,5'-Tetramethylbenzidine is 54827-17-7.

What is the molecular formula and weight of 3,3',5,5'-Tetramethylbenzidine?

The molecular formula of 3,3',5,5'-Tetramethylbenzidine is C16H20N2 and its molar mass is 240.35 g/mol.

What is 3,3',5,5'-Tetramethylbenzidine used for?

Clinical reagent for testing blood.

Is 3,3',5,5'-Tetramethylbenzidine hazardous?

3,3',5,5'-Tetramethylbenzidine carries the GHS signal word "Warning". Main hazards: harmful if swallowed, causes skin irritation, causes serious eye irritation. This summary is not an SDS, always read the supplier safety data sheet.

What is the HS code of 3,3',5,5'-Tetramethylbenzidine?

3,3',5,5'-Tetramethylbenzidine is usually classified under HS code 292159 (Aromatic polyamines and their derivatives, salts thereof, other). This is indicative, confirm it with your customs broker before shipping.

Where can I buy 3,3',5,5'-Tetramethylbenzidine?

CompoundList lists 96 suppliers of 3,3',5,5'-Tetramethylbenzidine in China, United States, Germany, United Kingdom and 10 more. Send one free request to get and compare quotes. Your contact details stay private until you accept a quote.

Where can I buy 3,3',5,5'-Tetramethylbenzidine in India?

2 suppliers in India list 3,3',5,5'-Tetramethylbenzidine on CompoundList. Filter the suppliers list by country and send a request to compare prices, grades and delivery times.

What is the price of 3,3',5,5'-Tetramethylbenzidine?

Suppliers quote 3,3',5,5'-Tetramethylbenzidine on request because prices depend on grade, purity, quantity and destination. A free request on CompoundList gets you several quotes to compare.

Guides for buyers

Practical guides that help when you source, ship or handle 3,3',5,5'-Tetramethylbenzidine

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Data last updated 7 Oct 2026. Details may have changed, please confirm them with the supplier. Report an error on this page
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