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Basic and cationic dyes

C.I. Basic Yellow 2

Also known as Auramine O, Basic Yellow 2, Auramine hydrochloride

CAS 2465-27-2Formula C17H22ClN3Molar mass 303.84 g/molEC 219-567-2
GHS signal word: Danger. Pictograms: Acute toxicity (severe), Harmful or irritant, Serious health hazard, Hazardous to the environment 61 suppliers listed
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C.I. Basic Yellow 2 chemical structure, C17H22ClN3, CAS 2465-27-2
2D chemical structure

Key facts

What buyers check first

All properties
Appearance
Yellow powder
Melting point
>250
Solubility
10 mg/ml in water, 60 mg/ml in ethylene glycol methyl ether, 20 mg/ml in EtOH
HS code
320413
Category
Basic and cationic dyes
Illustrative photo, yellow pigment powder in an open kraft paper sack in a chemical plant
Typical packagingIllustrative packaging image, not the actual product

What is C.I. Basic Yellow 2?

Plain description, key identifiers and synonyms

Auramine O is a hydrochloride obtained by combining 4,4'-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. A fluorescent stain for demonstrating acid fast organisms in a method similar to the Ziehl Neelsen. It also can be used to make a fluorescent Schiff reagent. It has a role as a fluorochrome and a histological dye. It contains an auramine O(1+).

Identifiers

Registry numbers, names and line notations, tap to copy

IUPAC name
4-[4-(dimethylamino)benzenecarboximidoyl]-N,N-dimethylaniline, hydrochloride
CAS number
2465-27-2 CAS number cross-checked
EC numbers
219-567-2, 682-593-1
Molecular formula
C17H22ClN3
UNII
DL992L7W39
MDL number
MFCD00012484
InChIKey
KSCQDDRPFHTIRL-UHFFFAOYSA-N
InChI
InChI=1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
SMILES (canonical and isomeric)
CN(C)C1=CC=C(C=C1)C(=N)C2=CC=C(C=C2)N(C)C.Cl

Synonyms

  • Auramine O
  • Basic Yellow 2
  • Auramine hydrochloride
  • Aizen auramine
  • Mitsui Auramine O
  • Auramine Yellow
  • Auramine Extra
  • Auramine Pure
  • Calcozine Yellow OX
  • Auramine N
  • Auramine chloride
  • Auramine FA
  • Auramine II
  • Auramine ON
  • Auramine OO
  • Auramine OS
  • Auramine SP
  • Auramine FWA
  • Auramine OOO
  • Adc Auramine O
  • Auramine A1
  • Aizen auramine OH
  • CI Basic Yellow 2
  • Auramine 0-100
  • Zlut zasadita 2
Show all 39 synonyms
  • Auramine Lake Yellow O
  • Aizen Auramine conc.SFA
  • Auramine Extra Conc. A
  • Basic Yellow 2, fugitive
  • Aizen Auramine Conc. SFA
  • C.I. Basic Yellow 2, Monohydrochloride
  • Auramine O (biological stain)
  • Auramine O Extra Conc. A Export
  • (Tetramethyldiamino)diphenylketoimine hydrochloride
  • C.I. 41000
  • 1,1-Bis(p-dimethylaminophenyl)methylenimine hydrochloride
  • CI 41000
  • Benzenamine, 4,4'-carbonimidoylbis[N,N-dimethyl-, monohydrochloride
  • CI Basic Yellow 2, monohydrochloride

Physical and chemical properties

Measured values and descriptors computed from the structure

Physical and chemical properties

Measured values, check the supplier specification for your grade

Appearance
Yellow powderexp.
Melting point
>250exp.
Solubility
10 mg/ml in water, 60 mg/ml in ethylene glycol methyl ether, 20 mg/ml in EtOHexp.

Values vary by grade. Ask suppliers for the COA or TDS of the batch you buy.

Computed descriptors

Calculated from the structure, useful for screening and formulation

Molecular weight
303.84 g/molcalc.
Exact mass
303.1502 Dacalc.
LogP (octanol/water)
3.66calc.
Topological polar surface area
30.33 Ųcalc.
Hydrogen bond donors
1calc.
Hydrogen bond acceptors
3calc.
Rotatable bonds
4calc.
Heavy atoms
21calc.
Rings
2calc.
Aromatic rings
2calc.
Fraction sp3 carbons
0.24calc.
Formal charge
0calc.
Stereocentres
0calc.
Components
2calc.
Lipinski rule of five
Passes (0 violations)calc.

Calculated for screening. Confirm critical figures with the supplier.

Murcko scaffold

The ring system that remains when every side chain is removed

Murcko scaffold of this chemical, N=C(c1ccccc1)c1ccccc1

N=C(c1ccccc1)c1ccccc1

2 rings, 14 atoms, the Benzophenone imine ring system.

548 other chemicals share this scaffold

Safety and hazards

GHS classification summary, not a safety data sheet

GHS signal word
Danger
GHS06: Acute toxicity (severe)
Acute toxicity (severe)
GHS07: Harmful or irritant
Harmful or irritant
GHS08: Serious health hazard
Serious health hazard
GHS09: Hazardous to the environment
Hazardous to the environment

Hazard statements

  • H302Harmful if swallowed.
  • H311Toxic in contact with skin.
  • H319Causes serious eye irritation.
  • H351Suspected of causing cancer.
  • H411Toxic to aquatic life with long lasting effects.
Precautionary statements (19)
  • P203Obtain, read and follow all safety instructions before use.
  • P262Do not get in eyes, on skin, or on clothing.
  • P264Wash skin thoroughly after handling.
  • P264 + P265Wash skin thoroughly after handling. Do not touch eyes.
  • P270Do not eat, drink or smoke when using this product.
  • P273Avoid release to the environment.
  • P280Wear protective gloves, protective clothing, eye protection and face protection.
  • P301 + P317If swallowed: Get medical help.
  • P302 + P352If on skin: Wash with plenty of water.
  • P305 + P351 + P338If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P316Get emergency medical help immediately.
  • P318If exposed or concerned, get medical advice.
  • P321Specific treatment (see supplemental first aid instructions on the label).
  • P330Rinse mouth.
  • P337 + P317If eye irritation persists: Get medical help.
  • P361 + P364Take off immediately all contaminated clothing. And wash it before reuse.
  • P391Collect spillage.
  • P405Store locked up.
  • P501Dispose of contents and container in accordance with local regulations.

GHS classification summary, not a safety data sheet (SDS). Classifications can differ by supplier, grade and concentration. Always check the supplier's SDS.

Full safety data and first aid

C.I. Basic Yellow 2 suppliers

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All 61 suppliers
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Where suppliers are based

61 suppliers in 7 countries

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C.I. Basic Yellow 2 price

Indicative price range per kg from live supplier offers

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Uses and applications

What the substance is used for, by industry and function

Auramine hydrochloride has an affinity for a wide spectrum of fibers. In fact, it has been used to dye materials as diverse as paper, leather, cotton, wool, silk, jute and coco fiber. It is an excellent fluorochrome and is used in fluorescent staining of acid-fast bacteria, tissue stain.

Roles

  • dye
  • fluorescent dye
  • fluorochrome
  • histological dye

How it is made

Made by heating Michler's ketone (4,4'-bis(dimethylamino)benzophenone) with ammonium chloride.

Regulatory status

Inventories, regulations and trade controls that list this substance

United States

  • TSCA inventory (USA)
    Status: Active in US commerce

HS code and trade data

Customs classification and reported world trade statistics

Global trade

World import and export statistics for the HS code C.I. Basic Yellow 2 is traded under.

HS 3204.13Indicative HS code

Basic dyes and preparations based thereon

World exports in 2024
$209M
−2.6% vs 2023, imports $224M
2019 to 2024
Top exporters
  1. India * partner reported27.9%
  2. China21.8%
  3. Spain6.6%
Top importers
  1. United States11.2%
  2. China7.9%
  3. Germany7.4%

* Partner reported figures, a lower bound.

Reported customs values in USD, not prices.Full trade data

The HS code shown for C.I. Basic Yellow 2 is indicative (it was assigned by structure rules). Confirm the classification with your customs broker before shipping.

Frequently asked questions

Quick answers about C.I. Basic Yellow 2, based on the data on this page

What is C.I. Basic Yellow 2?

Auramine O is a hydrochloride obtained by combining 4,4'-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. A fluorescent stain for demonstrating acid fast organisms in a method similar to the Ziehl Neelsen. It also can be used to make a fluorescent Schiff reagent.

What is the CAS number of C.I. Basic Yellow 2?

The CAS registry number of C.I. Basic Yellow 2 is 2465-27-2.

What is the molecular formula and weight of C.I. Basic Yellow 2?

The molecular formula of C.I. Basic Yellow 2 is C17H22ClN3 and its molar mass is 303.84 g/mol.

What is C.I. Basic Yellow 2 used for?

Auramine hydrochloride has an affinity for a wide spectrum of fibers. In fact, it has been used to dye materials as diverse as paper, leather, cotton, wool, silk, jute and coco fiber. It is an excellent fluorochrome and is used in fluorescent staining of acid-fast bacteria, tissue stain.

Is C.I. Basic Yellow 2 hazardous?

C.I. Basic Yellow 2 carries the GHS signal word "Danger". Main hazards: harmful if swallowed, toxic in contact with skin, causes serious eye irritation. This summary is not an SDS, always read the supplier safety data sheet.

What is the HS code of C.I. Basic Yellow 2?

C.I. Basic Yellow 2 is usually classified under HS code 320413 (Basic dyes and preparations based thereon). This is indicative, confirm it with your customs broker before shipping.

Where can I buy C.I. Basic Yellow 2?

CompoundList lists 61 suppliers of C.I. Basic Yellow 2 in India, China, United States, United Kingdom and 3 more. Send one free request to get and compare quotes. Your contact details stay private until you accept a quote.

Where can I buy C.I. Basic Yellow 2 in India?

28 suppliers in India list C.I. Basic Yellow 2 on CompoundList. Filter the suppliers list by country and send a request to compare prices, grades and delivery times.

What is the price of C.I. Basic Yellow 2?

Suppliers quote C.I. Basic Yellow 2 on request because prices depend on grade, purity, quantity and destination. A free request on CompoundList gets you several quotes to compare.

Guides for buyers

Practical guides that help when you source, ship or handle C.I. Basic Yellow 2

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Data last updated 7 Oct 2026. Details may have changed, please confirm them with the supplier. Report an error on this page
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